The investigator will examine the effect of a single dose of pyridostigmine, AchE inhibitor
on insulin secretion in healthy subjects. Each subject will undergo an intravenous glucose
tolerance test (IVGTT) where IV glucose will be administered and the glucose excursion and
insulin secretion response will be evaluated by measuring insulin and glucose at different
time points. The test will be carried out twice, once without and once with the
administration of a single dose of Pyridostigmine on two separate days. The investigator
hypothesizes that inhibiting AChE will potentiate insulin secretion.