Antimicrobial Activity of Two Mouthwashes

• Status: Completed
• Trial end date: 2015-12-01
• Target enrollment: 0
• Participant gender: All

Summary

This is a three arm randomized trial. The aim is to evaluate the antimicrobial activity of chlorhexidine and polyhexamethylene biguanide oral antiseptics on the microorganisms of the oral cavity. A total of 30 healthy volunteers will be enrolled and randomly allocated to control group (CG, n=10), which will be instructed to rinse the mouth with 10ml of a sterile saline solution for one minute, to chlorhexidine group (ClG, n=10), which will be instructed to rinse the mouth with 10ml of an 0.12% chlorhexidine solution, for one minute, or to polyhexamethylene biguanide group (PG, n=10), which will be instructed to rinse the mouth with 10ml of an 0.07% polyhexamethylene biguanide solution, for one minute. Samples of saliva will be collected before the mouth wash and after 30, 60 and 180 minutes. Samples will be plated on manitol agar, mitis salivarius agar, EMB agar and Sabouraud agar. Samples will be processed by a blinded microbiologist.

• Phase: N/A
• Accepts Healthy Volunteers?: Accepts Healthy Volunteers

Details

• Lead Sponsor: Universidade do Vale do Sapucai
• Treatments: Anti-Infective Agents; Biguanides; Chlorhexidine; Chlorhexidine gluconate; Pharmaceutical Solutions; Polihexanide; Polyhexamethylene biguanide

Criteria

Inclusion Criteria:

• patients aged 18-30 years

Exclusion Criteria:

• patients with a hypersensitivity or allergic response by some component of the formula antiseptics,

• pregnant women,

• lactating,

• history of periodontal disease,

• smoking,

• decompensated diabetics,

• patients undergoing chemotherapy,

• radiotherapy patient,

• transplanted patient and refuses to participate,

• not signing a term of free and informed concentiment