Changes in Resistome After Dental Extraction and Amoxicillin.

• Status: Unknown status
• Trial end date: 2018-03-01
• Target enrollment: 0
• Participant gender: All

Summary

The aim of this study is to evaluate the nature of the change in oral and gut bacterial resistance profiles after antibiotic therapy for a surgical procedure in two groups. The intervention group will receive a course of amoxicillin and analgesics after surgical extraction and have bacterial samples taken from saliva, tongue coatings and stool samples at four appointments over a period of six months. This will be compared to the control group which receive only analgesics after the surgical extraction.

An examination of the development and sustainability of antibiotic resistance in the oral and gut microbiome of healthy cohorts will be followed up for 6 months, after surgical extraction of impacted teeth.

Change in proportion of antibiotic resistant bacterial components will be studied using Metagenomic DNA sequencing and quantification of resistant genes .

• Accepts Healthy Volunteers?: Accepts Healthy Volunteers

Details

• Lead Sponsor: The University of Hong Kong
• Treatments: Amoxicillin; Anti-Bacterial Agents; Antibiotics, Antitubercular

Criteria

Inclusion Criteria:

1. Men and women aged between 18 and 45 years

2. Subjects who are scheduled to have a surgical tooth extraction.

3. Subjects who have signed an informed consent after being informed of the study design, objectives and, risks/benefits

4. No history of use of antibiotics up to three months prior to participation in the study.

5. No medical condition that prevents the surgical procedure or antibiotic therapy.

6. No history of hypersensitivity to beta lactams.

7. No contraindication to amoxicillin therapy.

8. Subjects are not pregnant or currently breast feeding.

9. No known or suspected immunodeficiency.

Exclusion Criteria:

1. History of antibiotic use within 3 months from baseline evaluation.

2. Any medical condition that prevents the surgical procedure or antibiotic therapy.

3. History of hypersensitivity to beta lactams

4. Contraindication for amoxicillin therapy

5. Pregnant or breast feeding women.

6. Subjects with known or suspected immunodeficiency.

7. Caries active subjects defined as subjects with active caries involving more than 3 teeth.

8. Subjects diagnosed with chronic periodontitis.

9. Subjects regularly using an antibacterial mouth rinse or planning to use one after the extraction.