Overview

Distinct Response of CD4+CD25+Foxp3+ and IL-10-secreting Type I T Regulatory Cells to Cluster Specific Immunotherapy in Allergic Rhinitis Children

Status:
Completed
Trial end date:
2010-10-01
Target enrollment:
0
Participant gender:
All
Summary
While allergen specific immunotherapy (SIT) is highly effective for allergic diseases in children, the underlying immunological mechanisms are unclear. Regulatory T (Treg) cells may be crucial in induction of tolerance. Our aim was to investigate the role of CD4+CD25+Foxp3+ T cells and IL-10-secreting type I T regulatory (Tr1) cells in the response to one year of cluster SIT to Dermatophagoides pteronyssinus for allergic rhinitis in children. CD4+CD25+Foxp3+regulatory T cells and IL-10-secreting type I T regulatory (Tr1) cells were analyzed in children allergic to Dermatophagoides pteronyssinus during one year cluster specific immunotherapy (SIT) in a prospective and randomized study. Peripheral blood mononuclear cells (PBMCs) were collected from 25 children receiving SIT and 21 receiving pharmacotherapy. The frequencies of CD4+CD25+Foxp3+ T cells and allergen-specific IL-10+IL-4-, IFN-γ+IL-4-, IL-4+IFN-γ-CD4+ T cells were measured by flow cytometry. Production of IL-4, IFN-r, and IL-10 in supernatants from allergen-stimulated PBMC culture was measured by ELISA. Finally, the suppressive effect of CD4+CD25highTreg cells from both groups was estimated.
Phase:
N/A
Accepts Healthy Volunteers?
No
Details
Lead Sponsor:
Beijing Tongren Hospital
Treatments:
Histamine Antagonists
Histamine H1 Antagonists
Criteria
Inclusion Criteria:

- Clinical diagnosis of Allergic rhinitis

- Han nationality

- Had a history of HDM-induced moderate or severe rhino-conjunctivitis of at least three
years duration

- Had a positive skin prick test (SPT) result for Der p (ALK-Abello', Hørsholm, Denmark)
with a wheal diameter of at least 6 mm

- Were positive for specific immunoglobulin E (IgE) to Der p (Pharmacia CAP System,
Pharmacia Diagnostics, Uppsala, Sweden), with a RAST value of at least 0.7 kU/L

Exclusion Criteria:

- Clinical diagnosis of asthma