Effect of Propofol and Sevoflurane Anesthesia on HSP70 Expression in Tumor Cells
Status:
ACTIVE_NOT_RECRUITING
Trial end date:
2025-12-30
Target enrollment:
Participant gender:
Summary
The goal of this clinical trial is to learn if sevoflurane-based inhalational anesthesia (s-IA) and propofol-based total intravenous anesthesia (p-TVA) have different effects on HSP70 expression in different types of tumour cells. The main questions it aims to answer are:
* if p-TVA/s-IA increase or suppress HSP70 expression in tumor cells in comparison with a pre-anesthesia level;
* does this change in HSP70 expression cause any difference in vital characteristics of tumor cells, such as proliferation, apoptosis, colony formation and migration.
Researchers will compare p-TVA with s-IA by ability of these types of anesthesia to change the HSP70 expression level and modulate HSP70-mediated effects in tumor cells.
Participants will:
* be randomly allocated to p-TVA or s-IA groups;
* donate 12 ml of blood before anesthesia induction and 12 ml after 2 hours of anesthesia.
The blood serum will be used to prepare cell medium. After exposure to this medium, cells from different tumor types will be investigated using cytological and molecular biological methods.
Phase:
EARLY_PHASE1
Details
Lead Sponsor:
Saint Petersburg State University, Russia
Collaborators:
Institute of Cytology of the Russian Academy of Sciences The Russian Science Foundation