Effects of Azithromycin as an Adjunct to Scaling and Root Planning in the Treatment of Periodontitis
Status:
Completed
Trial end date:
2019-06-28
Target enrollment:
Participant gender:
Summary
The growing interest in the possibilities of modulating macrophages in inflammatory diseases
with therapeutic purpose has prompted the development of new approaches for the treatment of
periodontitis.
This randomized add-on open clinical study evaluated the short-term effects of azithromycin
(Az) as an adjunct to scaling and root planing (SRP) in patients with chronic periodontitis.
The investigators measured periodontal parameters, and collected gingival biopsies from
patients with periodontitis (P group, n=50) before and 1 month after scaling and root
planning (SRP group, n=25), after scaling and root planning with oral azithromycin
administration (Az group, n=25), and from periodontally healthy individuals (H group, n=25).
The last served as a reference group. Macrophage subpopulations were identified through
immunohistochemistry as single positive CD68+ and CD163+ cells. The levels of M1-, and
M2-related cytokines (IL1-β, IL-6, IL-10, TGF-β) assay in the tissue culture medium was
provided by ELISA. The data were statistically analyzed by appropriate methods.
The null hypothesis tested was that L-arginine and L-ornithine have no influences on CD68+
and CD163+ Mφs densities.