Effects of Nutritional Fat on the Growth of Intestinal E. Coli
Status:
Unknown status
Trial end date:
2019-09-24
Target enrollment:
Participant gender:
Summary
Recent experiments in the lab of Prof. WD Hardt revealed, that in mice, 24 h exposure to a
high-fat diet results in a breakdown of colonization resistance against Salmonella
typhimurium. Mechanistic experiments identified bile acids as the mediator for reduced
colonization resistance. Exposure to a high fat diet leads to increased bile acid secretion
which in turn modify the intestinal microbiota.
It is now the aim to verify the results of this study in human healthy volunteers. The
nutritional habits of all participants will carefully be evaluated. In the intervention
phase, participants will be exposed to either high-fat or low-fat diet and a controlled dose
of the non-pathogenic bacteria E. coli Nissle. E. coli Nissle is the active compound for
"Mutaflor®" and other probiotics.
It is planned to enumerate E. coli Nissle counts in the stool after Mutaflor ingestion and to
quantify other changes of the human microbiota. The hypothesis is that a high-fat diet leads
to increased bile acid secretion results in favorable growth conditions for E. coli Nissle,
resulting in high bacterial counts in the stool.