Ghrelin Effect on Beta Cell Function in Health and Disease

• Status: Unknown status
• Trial end date: 2014-04-01
• Target enrollment: 0
• Participant gender: All

Summary

To determine the role of nutrient status on ghrelin regulation of insulin secretion. We hypothesize that ghrelin and glucagon-like peptide-1 (GLP-1)(both are hormones made in the gut,) have differential effects on β-cell function in the fed state. We will compare insulin secretion and glucose turnover during meal ingestion using a dual glucose tracer and mixed meal protocol in subjects receiving ghrelin or saline. We will also determine the role of ghrelin-stimulated GLP-1 levels in this process using the GLP-1 receptor (GLP-1R) antagonist Exendin(9-39) (Ex-9).

• Phase: N/A
• Accepts Healthy Volunteers?: Accepts Healthy Volunteers

Details

• Lead Sponsor: David Dalessio
• Collaborator: National Institutes of Health (NIH)

Criteria

Inclusion Criteria:

1. Healthy men and women

2. Ages between 18 and 45 years

3. BMI between 18 and 29 kg/m2

Exclusion Criteria:

1. History or clinical evidence of impaired fasting glucose or diabetes mellitus, myocardial infarction within the past year, history or symptoms of congestive heart failure, uncontrolled hypertension, history or active liver or renal disease (AST or ALT >2x upper limits of normal, calculated glomerular filtration rate [GFR] <60).

2. History of pituitary or adrenal disorders or neuroendocrine tumor.

3. Anemia defined as hematocrit <33%.

4. Use of medications that alter glucose metabolism

5. Pregnancy or lactation.

6. Abnormal Electrocardiogram (ECG): evidence of ischemia or arrhythmia.

7. Women who have a positive pregnancy test at any time during the study period.