Identification of Response to Hypertonic Dextrose Prolotherapy Markers in Knee Osteoarthritis Patients by Cytokine Array
Status:
Completed
Trial end date:
2017-05-26
Target enrollment:
Participant gender:
Summary
Hypertonic dextrose prolotherapy has been used in knee osteoarthritis (OA) clinically for a
long time. However the study about the mechanism is scant. The question of biomarkers of knee
OA cartilage response to hypertonic dextrose prolotherapy remains currently unresolved. On
this basis, the aim of this study was to characterize the secreted protein factors behind the
inflammatory potential involving the hypertonic dextrose prolotherapy of knee OA.
10~12 older than 65 years old knee OA patients will be recruited for clinical Western Ontario
McMaster University Osteoarthritis (WOMAC) index, knee X ray evaluation and knee joint
synovial fluid analysis before and after hypertonic dextrose prolotherapy. The expressions of
inflammatory factors will be measured by a novel cytokine antibody array methodology. The
study will evaluate inflammation-related cytokines in patients of knee joint synovial fluid.
Human Cytokine Antibody Array that allows profiling synovial fluid production of anti and
pro-inflammatory cytokines simultaneously. Altered levels of cytokine from the array
membranes incubated with tissue lysates will quantitatively depict as a histogram for
relative cytokine induction or reduction in the synovial fluid. The cytokines messenger
ribonucleic acid levels will be quantified by quantitative reverse-transcription polymerase
chain reaction and proteins expression was analyzed by Enzyme-Linked ImmunoSorbent Assay. The
WOMAC Index, minimum joint space width, and Human Cytokine Antibody Array between before and
after interventions will be compared. Differences between groups will be determined by the
Mann-Whitney U test. For the synovial angiogenesis is accompanied with inflammatory feature
in knee OA, associations between endothelial growth factor (VEGF) expression and VEGF
isoforms pattern will be determined with the Spearman correlation.
Phase:
N/A
Details
Lead Sponsor:
National Yang Ming Chiao Tung University Hospital National Yang-Ming University Hospital