Immediate Effect and Substantivity of the Essential Oils Without Alcohol on the Oral Biofilm in Situ
Status:
Unknown status
Trial end date:
1969-12-31
Target enrollment:
Participant gender:
Summary
This study aims to evaluate the in situ antibacterial activity of a mouthwash containing
essential oils with and without alcohol on undisturbed de novo plaque-like biofilm
(PL-biofilm) up to 7 h after its application.
An appliance was designed to hold six glass disks on the buccal sides of the lower teeth,
allowing PL-biofilm growth. Twenty healthy volunteers will the appliance for 48 h and then
performed a mouthwash with essential oils. Disks will be removed after 30 s and at 1, 3, 5,
and 7 h later. After a washout period, the same procedure will be repeated with a sterile
water mouthwash and a mouthwash with essential oils without alcohol. After PL-biofilm vital
staining, samples will be analyzed using a confocal laser scanning microscope analysing their
bacterial viability and thickness.