LC-MS/MS Based Method Development for the Monitoring of Antibiotic Concentrations in Sputum of Cystic Fibrosis Patients
Status:
Suspended
Trial end date:
2020-12-01
Target enrollment:
Participant gender:
Summary
In this trial, various factors that may influence the antibiotic concentrations measured in
the sputum of cystic fibrosis patients are studied.
A first factor is aerosol use. As cystic fibrosis patients often use aerosols, such as
hypertonic saline, dilution of the antibiotics in sputum can be expected. The extent of this
dilution is unknown and will be determined by comparing sputum samples collected before and
after the use of an aerosol.
A second factor is the homogeneity of the antibiotics within one sputum sample. Multiple
aliquots of the same sputum sample will be compared.
A third factor is the variability between several sputum samples collected during a drainage
session. The antibiotic concentrations in 3 separate sputum samples will be compared.
The final goal is to standardise the sputum sample collection and processing of the samples
to ensure a accurate concentration measurements in sputum.
Phase:
N/A
Details
Lead Sponsor:
University Ghent
Collaborators:
Belgische Vereniging voor Strijd tegen Mucoviscidose King Baudouin Foundation