Platelet-rich Plasma and Synovial Cytokines in Knee Osteoarthritis

• Status: Completed
• Trial end date: 2020-03-14
• Target enrollment: 0
• Participant gender: All

Summary

A prospective study was performed on 90 patients were included and categorized into mild (30 cases, moderate (30 cases) and severe (30 cases) knee OA. Three intra-articular (I.A) injections of PRP, 2 weeks a part, were received. Pain score and MRI Osteoarthritis Knee Score (MOAKS) were assessed. Serial synovial fluid cytokines assays in the form of Tumor necrosis factor-α (TNF-α) and Macrophage migration inhibitory factor (MIF), were performed using commercially available ELISA assay kits. The assays were performed pre-injection (S1), two weeks from the 1st I.A injection and two weeks from the 2nd I.A injection (S3) for all included patients.

• Phase: N/A
• Accepts Healthy Volunteers?: No

Details

• Lead Sponsor: South Valley University

Criteria

Inclusion Criteria:

• All patients have unilateral knee OA

Exclusion Criteria:

• Polyarticular disease.

• Knee arthroscopy in the previous year.

• HA or steroid IA penetration in the preceding 3 months.

• History of infectious disease and autoimmune disorders such as diabetes, rheumatoid arthritis.

• Hematologic diseases (coagulopathy).

• Serious cardiovascular diseases , infections or immunodepression.

• Anticoagulant therapy or an anti-aggregating agent.

• Uuse of non-steroidal anti-inflammatory drugs 2 weeks prior to blood sampling.

• < 10 g / dL of hemoglobin