Proteomics & Glyco-Proteomic Analysis of Follicular Fluid
Status:
Unknown status
Trial end date:
2014-01-01
Target enrollment:
Participant gender:
Summary
To the best of the investigators knowledge, exhaustive characterization of the low and high
abundant proteins and glyco-proteins of the Follicular Fluid (FF) has not yet been achieved.
Such an analysis may provide critical molecular data on the role of the FF in oocyte
maturation and may identify specific changes in the FF proteome of patients with gynecologic
problems, such as Polycystic Ovary Syndrome (PCOS).
Specific Aims
1. To perform a comprehensive analysis of normal human FF using sensitive mass spectrometry
in combination with conventional approaches for proteomic evaluation and using HPLC and
Western blot for glyco-proteomic analysis.
2. Characterize differential proteomic and glyco-proteomic patterns of the FF in normal
women compared to lean and obese women with PCOS.
3. To supplement the differential proteomic and glyco-proteomic analysis with steroid
hormone analysis in all FF samples.