Proteomics for Identification of Hyperoxia-induced Changes in Protein Expression

• Status: Unknown status
• Trial end date: 2016-12-01
• Target enrollment: 0
• Participant gender: All

Summary

Aim of the present study is to investigate the influence of hyperoxia on the protein expression using the differential analysis of protein expression in tissues (proteomics). In the study, blood and urine samples will be collected from participants who undergo a short term hyperoxia using 100% oxgen for 3 hours.

Here, gel electrophoresis, protein separation and mass spectroscopy allow to identify affected proteins. Based on these results, different induction factors of proteins will be determined and then assessed using a bioinformatic network analysis regarding the cellular influence.

• Phase: N/A
• Accepts Healthy Volunteers?: Accepts Healthy Volunteers

Details

• Lead Sponsor: University Hospital of Cologne

Criteria

Inclusion Criteria:

• American Society of Anesthesiologists (ASA) 1

• >18 years

• < 50 years

Exclusion Criteria:

• American Society of Anesthesiologists (ASA) > 1

• pregnant

• <18 years

• > 50 years

• frequent or recent drug intake