Quantitation of Bacteria Associated With Severe Periodontitis by Real -Time PCR Following Application of Diode Laser

• Status: Completed
• Trial end date: 2021-12-01
• Target enrollment: 0
• Participant gender: All

Summary

1. Primary objective To measure the gingival crevicular levels of A.actinomycetemcomitans and P.gingivalis after intra-pocket application of diode laser in severe periodontitis (stage 3 grade C) patients.

2. Secondary objective To compare the levels of the same microorganisms as in cases of diode laser therapy to those after conventional treatment with systemic antibiotic administration in severe periodontitis (stage 3 grade C) patients.

• Phase: Phase 2/Phase 3
• Accepts Healthy Volunteers?: Accepts Healthy Volunteers

Details

• Lead Sponsor: Alexandria University

Criteria

Inclusion Criteria:

• (1) Patients of both sexes having severe periodontitis (stage 3 grade C) with interdental clinical attachment loss (CAL) ≥ 5.

(2) Patients' age between 15 and 35 years old with rapid bone loss showed by radiograph.(3) Systemically healthy patients.

(4) Radiographic bone loss extending to middle or apical third of root. (5) Probing depth (PD) ≥ 6 mm.

Exclusion Criteria:

• (1) Use of antibiotics during the last 3 months. (2) Pregnancy. (3) Any systemic condition that might affect the study. (4) Use of mouthwash containing antimicrobials during the previous 3 weeks. (5) Smoking.