Supplementation With L-ornithine But Not L-arginine Increases Density of CD68+ and CD163+ Macrophages in Periodontitis
Status:
Completed
Trial end date:
2018-11-01
Target enrollment:
Participant gender:
Summary
The aim of the study was to investigate whether oral administration of L-arginine or
L-ornithine could modulate local representation density and ratio of macrophages in
periodontitis-affected gingiva by using immunohistochemical detection of CD68+ and CD163+
macrophages in biopsies of the gingiva.
The null hypothesis tested was that L-arginine and L-ornithine have no influences on CD68+
and CD163+ macrophages densities when supplementing the treatment of periodontitis.
Materials and methods. 75 individuals with a diagnosis of generalized periodontitis at stages
II-III and grade B (38 women and 37 men, 51% and 49%, respectively) were included in the
study. Periodontitis was diagnosed by using the criteria of the Classification of Periodontal
and Peri-Implant Diseases and Conditions 2017. 25 patients received scaling and root planing
only; 25 patients additionally received L-arginine, and 25 - L-ornithine, according to
instructions available in Ukraine.
For the immunohistochemical study of paraffin-embedded sections, the gingival biopsy was
taken from 5 selected patients per group before treatment and after 1 month. CD68+ (cluster
of differentiation 68 positive) and CD163+ cells served as a morphological equivalent of M1,
M2 macrophages subpopulations, and their densities were calculated per 10000 μm2. Statistical
analysis was performed by adequate power methods.